TABLE 2.
Properties of wild-type CHO-K1, ZP109, and RnPEX12-transfected ZP109 (109P3) cellsa
| Cell line | Peroxisome | Catalase latency (%) | DHAP-ATase (%) | P12/UV (%) | P9OH/UV (%) |
|---|---|---|---|---|---|
| CHO-K1 | + | 64 | 100 | 83 | <0.01 |
| ZP109 | − | 1.4 | 13 | <0.01 | 92 |
| 109P3 | + | 66 | 220 | 73 | <0.01 |
a
Catalase latency represents peroxisomal catalase, calculated from Fig. 3A as described previously (57). Peroxisomal DHAP-ATase was expressed as a percentage relative to that of wild-type CHO-K1 cells. For determination of P12/UV or P9OH/UV resistance, 200 or 105 cells were inoculated into 60-mm-diameter dishes and selected (43). The numbers of colonies were counted in triplicate experiments and expressed as percentages of that of the unselected control.