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. 2024 Feb 14;626(8001):1125–1132. doi: 10.1038/s41586-024-07041-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — Local resolution estimation and gold-standard Fourier Shell Correlation (FSC) validation. Final cryo-EM maps for Structure 4 (a) 70 S/Msmeg1130, Structure 5 (b) 70 S/Rv2629, and Structure 6 (c) 70 S/Msmeg1130/EF-Tu(GDP), surface coloured by estimated local resolution as indicated in the heat map scale. Two orthogonal views are shown. The gold-standard FSC curves of each half-map (red), using a ‘soft mask’ excluding solvent and model-map (green), are plotted across resolution. Map and model validation were performed in Phenix 1.19.2.