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. 2024 Feb 14;626(8001):1125–1132. doi: 10.1038/s41586-024-07041-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — (a–f) Panel descriptions refer to the Ribosome/Balon/RaiA map (left), Ribosome/Balon/tRNA/mRNA map (centre) and Ribosome/Balon/EF-Tu map (right) as indicated at the top of the figure. (a–c) Final cryo-EM maps, surface coloured by estimated local resolution as indicated in the heatmap key. Two orthogonal views are shown to illustrate two opposing sides of the ribosome particle. (d–f) Angular distribution plot of particles in the final reconstructions, shown as a Mollweide projection. (g–i) Gold-standard Fourier shell correlation (FSC) curves for final maps generated by RELION post-processing. Masked (blue), unmasked (green), and phase-randomised masked (red) plots are shown.