Extended Data Fig. 4. Cryo-EM maps for molecules that bind ribosomes concurrently with Balon (ice shock).

The cryo-EM maps show ribosomal ligands in two distinct classes of ribosomes in our dataset, with panels on the left coloured by chain and panels on the right coloured by local resolution. The first class represents the ribosome complex that contains heterologous mRNA, heterologous peptidyl-tRNA, and Balon. The second class represents the ribosome complex that comprises Balon and the hibernation factor RaiA. The third class corresponds to ribosome particles that bind Balon in complex with EF-Tu. The three domains of Balon are labelled as N-domain (N-terminal domain), M-domain (middle domain) and C-domain (C-terminal domain).