Fig. 1.

Generation of CAO antisense (CAOas) plants in tobacco (Nicotiana tabacum). A Schematic representation of the construct used for the antisense expression of AtCAO in the tobacco plant. B PCR analysis revealed the integration of CaMV35S-AtCAO into tobacco plant’s genome. A likely, 1.7 kb DNA fragment, was observed in all the 5 transgenics used (listed above the data), when the PCR was done using 35S-promoter internal forward primer and AtCAO forward primer. Further, as expected, 1.7 kb band was absent in the wildtype (WT) genomic DNA. C Northern blot analysis of CAO of WT and CAOas tobacco plants (CAOas1, CAOas2, CAOas3) grown under 300 µmol photons m⁻² s⁻¹ light (natural sun light + metal halide lamps) for 30 days. D Phenotype of WT and CAOas plants grown for 30 days under 300 μmol photons m⁻² s⁻¹light