Representative macrophages obtained from day 11 EB cultures derived from ES clones transfected with wild-type and mutant cDNAs. MGG cytological stains for macrophages detected in PU.1+/− cultures and cultures transfected with wild-type (PU.1), Δ245-272, Δ141, Δ3-21, and Δ2-167 transgenes are shown. Magnifications: A and F, ×27; B to E, ×68.