FIG. 8.

Gene expression in day 11 EB cultures derived from ES transformants containing the Δ141-167, Δ129-141, and Δ118-132 cDNA transgenes. Four independent clones were analyzed for the Δ118-132 and Δ141-167 constructs, while three independent clones were analyzed for the Δ129-141 transgene. EB assays were harvested for total cellular RNA, and RT-PCR analyses for PU.1, M-CSFR, and HPRT transcripts were performed. While a 500-bp RT-PCR product was detected in J774.1 and PU.1^+/+ ES RNAs, PCR products in clones transfected with the Δ141-167 construct were smaller due to the deletion introduced into the PU.1 cDNA.