Fig. 5. mGluR2 signaling is required to prevent the initiation of strong calcium transients in SAC dendrites during inward motion.

a Same as Fig. 4a, but after applying LY341495. b As in Fig. 4c, normalized GCaMP6f responses at different distances from the soma along individual dendritic branches after applying LY341495. Each line represents data from a single branch. In LY341395, in the inward direction, large GCaMP6f responses are more frequently detected during imaging along the dendrite. c Summary of GCaMP6f responses (dF/F0) before and after LY341495 application. Control data is the same as in Fig. 4d; LY341395, n = 10 cells. Kolmogorov-Smirnov Test *p = 0.01, **p < 0.01. For each box, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The whiskers extend to the most extreme data points not considered outliers, and the outliers are plotted individually using the ‘+‘ symbol. d Paired comparison of outward GCaMP6f transient half width in the distal half of the dendrites before and after applying LY341495. Two-tailed paired t test, *p = 0.03. N = 10 cells. e Left: Example Vm traces of current clamp recordings of SAC during the moving bar stimulus before and after LY341495. Traces are from individual trials. Darker traces are example single trials for easier visualization. Right: Summary plots of the amplitude of depolarization and the area of the trace during the depolarizing window. N = 6 cells for control, N = 7 cells for LY341485. Data are presented as mean values ± SEM. Two-sided unpaired t test, Bonferroni correction was made for multiple comparisons. p = 0.3 (left) and p = 0.8 (right). Source data are provided as a Source Data file.