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. 2024 Feb 28;10:103. doi: 10.1038/s41420-024-01873-z

Fig. 3. Suppressing of CREB5 inhibits proliferation and self-renewal ability in GSCs.

Fig. 3

A RT-qPCR analysis showing CREB5 knockdown after transduction with shRNA in GSCs. B Cell proliferation shCREB5 treated GSCs. C Annexin-V/propidium iodide staining of GSCs after transduction with shCREB5. D Representative images showing cell spheres. Scale bars = 100 μm. E An in vitro limiting dilution assay using gradually decreasing cell seeding density shows the cell sphere forming ability of GSCs transduced with shCREB5. Data are means ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.