Table 2.
Neuronal markers expressed in Monocyte-Derived-Neuronal-like Cells (MDNCs).
| Neuronal Marker | Flow Cytometry | Immuno fluorescence | RT-PCR | Single cell mRNA sequencing | |
|---|---|---|---|---|---|
| 1 | Neurofilament | present | |||
| 2 | Nestin | present | present | present | |
| 3 | MAP-2 | present | present | ||
| 4 | β3-tubulin | absent | present | ||
| 5 | PSD-95 | present | |||
| 6 | GAD-67 | present | present | present | |
| 7 | NeuN | present | |||
| 8 | BLOC1S3 | present | |||
| 9 | vGLUT1 | absent | |||
| 10 | vGLUT2 | absent | |||
| 11 | NMDAR1 | present | |||
| 12 | NMDAR2B | absent | |||
| 13 | Glutaminase | present | |||
| 14 | GABA transporter 1 | absent | |||
| 15 |
GABA type B receptor subunit 1 |
absent | |||
| 16 |
GABA type B receptor subunit 2 |
absent | |||
| 17 | GAD-65 | absent | |||
| 18 | Tyrosine hydroxylase | absent | |||
| 19 | DAT | absent | |||
| 20 | FOXA2 | absent | |||
| 21 | Girk-2 | present | |||
| 22 | Nurr-1 | present | |||
| 23 | LMX1B | present | |||
| 24 | Tryptophan hydroxylase-1 | present | |||
| 25 | Tryptophan hydroxylase-2 | present | |||
| 26 | Serotonin transporter | present | |||
| 27 | PET-1 | absent | |||
| 28 | Cholinacetyltransferase | absent | |||
| 29 | VAChT | absent | |||
| 30 | Acetylcholinesterase | present | |||
| 31 |
Insulin enhancer protein |
absent | |||
| 32 |
Motor neurons and pancreas homeobox-1 |
absent | |||
| 33 | Neurexin-3 | present | |||
| 34 |
Synaptosome associated protein-25 |
present | |||
| 35 |
Synaptic vesicle glycoprotein-2A |
present | |||
| 36 |
Vesicle associated membrane protein-1 |
present | |||
| 37 | SHANK-2 | present | |||
| 38 | Synuclein alpha | present | |||
| 39 | Syntaxin-1A | present | |||
| 40 | SPIRE-1 | present | |||
| 41 | Tau | present | |||
| 42 | Growth associated protein-43 | present | |||
| 43 |
GABA type A receptor subunit β-3 |
present |