Table 1.

Immortalized cell lines that were tested for AiV-A1 infection. Cell lines were cultivated in the respective growth medium, as specified by ATCC, and infected in the correspondently adapted infection medium.

Cell line	Tissue	Growth medium	Infection medium
HeLa	Epithelial, human cervix	•DMEM (Gibco, 21,063–029) •10 % FBS (Sigma, F7524) •1 % Pen-Strep (Sigma, 15,140–122)	•DMEM (Gibco, 21,063–029) •2 % FBS (Sigma, F7524) •1 % Pen-Strep (Sigma, 15,140–122)
RKO	Epithelial, human colon
Vero	Epithelial, African green monkey kidney
A549	Epithelial, human lung	•Ham's F-12 K Medium (Gibco, 21,127–022) •10 % FBS (Sigma, F7524) •1 % Pen-Strep (Sigma, 15,140–122)	•Ham's F-12 K Medium (Gibco, 21,127–022) •2 % FBS (Sigma, F7524) •1 % Pen-Strep (Sigma, 15,140–122)
T84	Epithelial, human colon
Caco-2	Epithelial, human colon	•DMEM (Gibco, 21,063–029) •10 % FBS (Sigma, F7524) •mM NEAA (Lonza, 13–114E) •1 % Pen-Strep (Sigma, 15,140–122) •1 mM Pyruvate (Sigma, S8636)	•DMEM (Gibco, 21,063–029) •2 % FBS (Sigma, F7524) •mM NEAA (Lonza, 13–114E) •1 % Pen-Strep (Sigma, 15,140–122) •1 mM Pyruvate (Sigma, S8636)
HCEC 1CT	Epithelial, human colon	•DMEM and Medium 199 Earle's, 4 + 1 (Biochrom, F0435 and FG0615) •4 mM GlutaMAXTM-1 (100X), (Gibco, 35,050–038) •2 % Cosmic Calf Serum (Hyclone, SH30087) •20 ng/ml EGF (Sigma-Aldrich, E9644) •10 μg/ml Insulin (Sigma-Aldrich, I9278) •2 μg/ml Apo-Transferrin (Sigma-Aldrich, T2036) •5 nM Sodium-Selenite (Sigma-Aldrich, S5261) •1 μg/ml Hydrocortisone (Sigma-Aldrich, H0396) •1 % Pen-Strep (Sigma, 15,140–122)