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Nuclear localization of the top1-lacZ fusion protein in salivary glands detected by X-Gal staining for β-gal activity. Salivary glands from third-instar larvae of NF315 (a), 28-1 (b), and wild-type (c) flies were prepared as described in Materials and Methods. The β-gal activity is readily detected in the nuclei of NF315 transgenic animals and is shown as the dark staining. Note that the smaller nuclei of adjacent tissue also exhibit the activity (a). Scale bar (b), 100 μm.
