Table 2.

The 10 most cited articles in chemistry

Rank	Citations1	Title	Subject	Year	Author(s)	Reference
1	351,269	Protein measurement with the Folin phenol reagent	Biology lab technique	1951	Lowry OH, Rosebrough NJ, Farr AL & Randall RJ	J Biol Chem (1951) 193: 265–27
2	256,211	Cleavage of structural proteins during the assembly of the head of bacteriophage T4	Biology lab technique	1970	Laemmli UK	Nature ‏(1970) 227: ‏680–685
3	233,152	A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein–dye binding	Biology lab technique	1976	Bradford MM	Anal Biochem (1976) 72: 248–254 ‏
4	86,210	Development of the Colle-Salvetti correlation–energy formula into a functional of the electron density	Physical chemistry	1988	Lee C, Yang W, Parr R	Phys Rev B (1988) 37:‏ 785–789
5	76,325	A short history of SHELX	Crystallography	2008	Sheldrick GM	Acta Crystallogr A (2008) 64: 112–122
6	72,173	Density-functional thermochemistry. 3. The role of exact exchange	Physical chemistry	1993	Becke AD	J Chem Phys (1993) 98: 5648–5652
7	70,057	Basic local alignment search tool	Bioinformatics	1990	Altschul SF, Gish W, Miller W, Myers EW & Lipman DJ	J Mol Biol (1990) 215: 403–410
8	69,361	DNA sequencing with chain-terminating inhibitors	Biology lab technique	1977	Sanger F, Nicklen S, Coulson AR	Proc Natl Acad Sci USA (1977) 74: 5463–5467
9	66,822	Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol–chloroform extraction	Biology lab technique	1987	Chomczynski P, Sacchi N	Anal. Biochem. (1987) 162: 156–159
10	61,048	A simple method for the isolation and purification of total lipides from animal tissues	Biology lab technique	1957	Folch J, Lees M, Stanley G	J Biol Chem (1957) 226: 497–509

¹As of 04/08/2023, retrieved from Web of Science, Clarivate Analytics