Fig. 7.

Flow cytometry of JC-1 staining. a. Representative figure of 10,000 separate events. Flow cytometry dot-plots of JC-1 fluorescence to measure ΔΨm in control astrocytes (blue dots) and TSC astrocytes (orange dots). Three subpopulations of cells were identified in both control and TSC astrocytes: ‘PE high FITC low’, ‘PE low FITC low’, and ‘PE high FITC high’ fluorescence. b–d. Barplots showing the percentage of control and TSC astrocytes with a positive JC-1 fluorescence intensity signal. No differences were identified between control and TSC astrocytes in ‘PE low FITC low’ fluorescence signal. TSC astrocytes showed a significant increase in PE high FITC high fluorescence intensity, while they showed a significant reduction in PE high FITC low fluorescence signal. Data are expressed as mean ± SEM; p value: *p value ≤ 0.05; **p value ≤ 0.01; ***p value ≤ 0.001; ****p value ≤ 0.0001. Mann–Whitney U test were used to evaluate significance