TABLE 2.
Plasmids used in this study
| Plasmid | Descriptiona | Source |
|---|---|---|
| pET-His | E. coli expression vector with six-His tag | 10 |
| pBM272 | CEN, URA3, GAL1-GAL10 promoter | 27 |
| YIp357R | Integration vector, URA3, lacZ as reporter gene | 24 |
| pLC25 | pET-6xHis-REB1 | |
| pLC6 | YIp357R-(ABC) REB1′-lacZ | |
| pLC7 | YIp357R-(BC) REB1′-lacZ | |
| pLC8 | YIp357R-(AC) REB1′-lacZ | |
| pLC104 | YIp357R-(AB) REB1′-lacZ | |
| pLC131 | YIp357R-(A) REB1′-lacZ | |
| pLC130 | YIp357R-(B) REB1′-lacZ | |
| pLC9 | YIp357R-(C) REB1′-lacZ | |
| pLC128 | YIp357R-(ABC+75)-REB1′-lacZ | |
| pLC107 | YIp357R-(null)-lacZ | |
| pLC30 | pBM272-REB1 (URA3) | |
| pLC31 | pBM272-REB1 (URA3 is replaced by HIS3) | |
| pLC151 | CEN TRP1 RPL32′-GFP | |
| pLC151C | CEN TRP1 RPL32′-CGFP |
a
Reb1p sites are indicated for YIp357R plasmids.