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. 2024 Feb 15;14:1267967. doi: 10.3389/fendo.2023.1267967

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Copyright © 2024 Wang, Xu, Zhang, Yin, Pan, He, Li, Cheng, Zhu, Zhao, Huang and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expressions and activities of wild-type and mutant 17β-HSD3 enzymes. (A) Western blot of FLAG-tagged wild- type 17β-HSD3 and mutants. β-Actin was applied as the control and analyzed using an anti-β-actin antibody. (B) Enzyme activity of wild-type and mutants17β-HSD3. The ELISA analysis yielding the percentage of testosterone that was formed from the initially provided androstenedione. Data from three independent experiments and represented mean ± SEM. (n = 3, Means ± SEM, ****P < 0.0001).