Figure 4.
Expressions and activities of wild-type and mutant 17β-HSD3 enzymes. (A) Western blot of FLAG-tagged wild- type 17β-HSD3 and mutants. β-Actin was applied as the control and analyzed using an anti-β-actin antibody. (B) Enzyme activity of wild-type and mutants17β-HSD3. The ELISA analysis yielding the percentage of testosterone that was formed from the initially provided androstenedione. Data from three independent experiments and represented mean ± SEM. (n = 3, Means ± SEM, ****P < 0.0001).