Figure 3.

Impaired global Ca²⁺ response in CD8⁺ T cells from P2rx4 ^-/- and P2rx7 ^-/- mice. Ca²⁺ imaging of CD8⁺ T cells of WT, P2rx4 ^-/- and P2rx7 ^-/- mice was performed with the Ca²⁺ indicator Fura-2 for 8 min. (A) Cells were stimulated with monoclonal antibodies against CD3 (10µg/mL) after the first min of measurements. For each condition, 3 mice were used (N = 3 per group). Data are mean ± SEM, WT n = 217 cells, P2rx4 ^-/- n = 164 cells, P2rx7 ^-/- n = 135 cells. Results were analyzed using Kruskal-Wallis test and Dunnett’s multiple comparisons test (***p < 0.001, ****p < 0.0001). The data were quantified by analyzing the signal onset velocity (B), the time to reach 200 nM Ca²⁺ (C) and the area under the curve (D).