Figure 3.
Impaired global Ca2+ response in CD8+ T cells from P2rx4 -/- and P2rx7 -/- mice. Ca2+ imaging of CD8+ T cells of WT, P2rx4 -/- and P2rx7 -/- mice was performed with the Ca2+ indicator Fura-2 for 8 min. (A) Cells were stimulated with monoclonal antibodies against CD3 (10µg/mL) after the first min of measurements. For each condition, 3 mice were used (N = 3 per group). Data are mean ± SEM, WT n = 217 cells, P2rx4 -/- n = 164 cells, P2rx7 -/- n = 135 cells. Results were analyzed using Kruskal-Wallis test and Dunnett’s multiple comparisons test (***p < 0.001, ****p < 0.0001). The data were quantified by analyzing the signal onset velocity (B), the time to reach 200 nM Ca2+ (C) and the area under the curve (D).