Figure 6.

CPC and GL2 mediates the PROPEPs signals in root hair formation. (A) qRT-PCR analysis of CPC and GL2 mRNA levels in wild type (WT) roots treated with 10 nM Pep2 for 24 h. The expression level of the untreated control (0 h) was set to 1.0, and Pep2 treatment levels were normalized to the control level. Data are means ± SD (n =3 individual reactions). (B, C) qRT-PCR analysis of CPC (B) and GL2 (C) mRNA levels in 6-d-old WT and propep2 roots. The expression level in WT root was set to 1.0. Data are means ± SD (n =3 individual reactions). (D) The growth phenotype of root hairs in wild type (WT), cpc and gl2 mutant. Four-day-old plants were transplanted on half-strength Murashige and Skoog (MS) agar medium supplemented with or without 10 nm Pep2 for 48h. Bars = 200 μm. (E, F) Statistics of the root hair number (E) and root hair length (F) as in (D). Data are means ± SD (n = 15 roots per treatment). Asterisks in (A–C, E, F) indicate statistically significant differences compared with the control. (Tukey’s test; *p < 0.05, **p < 0.01).