Supplementary Fig. 1.

Pharmacological inhibition of ALK3 and ALK2 with LDN does not protect against APAP-induced hepatotoxicity. A. Representative blots with cleaved caspase 3 antibody and their quantification normalized with tubulin antibody as loading control. Data are expressed as percentage relative to the control group (VEH, 100%) and presented as mean ± SEM. B. mRNA levels of HMOX1 determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold increase relative to control condition (C-VEH, 1) and presented as mean ± SEM. Experimental conditions: Huh7 treated with the inhibitor LDN-193189 (LDN) (500 nM) 1 h prior to APAP (20 mM, A20) stimulation for 16 h (n ≥ 3 independent experiments). ****p < 0.0001 A²⁰vs. C.