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Post-treatment of Huh7 cells with DMH2 protects against hepatotoxicity induced by APAP. A. Cell viability determined by crystal violet staining. Data are represented as percentage relative to control group (C, 100%). Experimental conditions: Huh7 treated with APAP (20 mM, A20) following with DMH2 (10 μM) treatment 1 h after APAP both maintained for 16 h (n > 3 independent experiments). ****p < 0.0001 A20vs. C; ###p < 0.005 A20-DMH2 vs. A20-DMSO.
