Supplementary Fig. 4.

Evaluation of DMH2 effects against APAP-induced liver damage at later time points. A. Representative 20X and 40X images of 4-HNE IHC in liver sections and the corresponding quantification. Data are presented as mean ± SEM and expressed as arbitrary units (a.u.). B. GSH levels. Data are expressed as percentage relative to control group (C-DMSO, 100%) and presented as mean ± SEM. C. Representative blots with the indicated antibodies and the corresponding quantification. Data are presented as mean ± SEM and expressed as percentage relative to control group (C-DMSO, 100%) and presented as mean ± SEM. Experimental conditions: Mice i.p. Injected with vehicle (DMSO) or APAP (500 mg/kg), and 1 h later treated with DMH2 or the corresponding amount of DMSO in a single dose of 3 mg/kg. Animals were sacrificed 6 h after APAP administration (n = 5 animals per group). *p < 0.05 and ***p < 0.005, APAP vs. C; ^##p < 0.01, APAP-DMH2 vs. APAP-DMSO.