Fig. 3.

Effect of silencing of BMP type I receptors ALK3 and ALK2 on cellular viability on Huh7 human hepatocytes. A. mRNA levels of ALK2 (left) and ALK3 (right) determined by RT-qPCR and normalized to 36B4 gene expression. B. Representative blots with the indicated antibodies and the corresponding quantification. Data are expressed as fold increase relative to the control condition (ShControl, 1) and presented as mean ± SEM. C - D. mRNA levels of HAMP and ID1, respectively, determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold increase relative to the control condition (ShControl, 1) and presented as mean ± SEM. Experimental conditions: Huh7 silenced stable lines ShALK2 and ShALK3 and their control ShControl (ShC) treated (n ≥ 3 independent experiments). **p < 0.01, ***p < 0.005 and ****p < 0.0001, ShALK2 or ShALK3 vs. ShC. E. Cell viability determined by crystal violet staining. Data are represented as percentage relative to control group (C, 100%) and presented as mean ± SEM. Experimental conditions: Huh7 silenced stable lines ShALK2 and ShALK3 and their control ShControl (ShC) treated with APAP (20 mM, A20) for 16 h (n ≥ 3 independent experiments). **p < 0.01 and ****p < 0.0001, A20 vs. C; ^####p < 0.0001, A20-ShALK3 vs. A20-ShC. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)