Fig. 4.

Silencing of ALK3 protects against APAP-induced cell toxicity. A. Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are presented as percentage relative to the positive control (100%). B. Representative blots with the indicated antibodies and the corresponding quantification. Data are expressed as percentage relative to the control group (ShControl, 100%) and presented as mean ± SEM. C. ROS production detected with DHE probe represented as DHE fluorescence F/F₀ (a.u.) through exposure to APAP during 2 h. D. mRNA levels of HMOX1, GSTM3 and SOD2 determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold increase relative to the control condition (ShControl 1) and presented as mean ± SEM. E. Representative blots with the indicated antibodies and the corresponding quantifications. Data are expressed as percentage relative to control group (ShControl, 100%) and presented as mean ± SEM. Experimental conditions: Huh7 silenced stable line ShALK3 and its control ShControl (ShC) treated with APAP 20 mM (A20) for 2 (C), 6 (E) or 16 (A, B, D) hours (n ≥ 3 independent experiments). *p < 0.05, **p < 0.01 and ****p < 0.0001, A20 vs. C, or ShALK3 vs. ShC; ^#p < 0.05, ^###p < 0.005 and ^####p < 0.001, C-ShALK3 vs. C-ShC or A20-ShALK3 vs. A20-ShC.