Fig. 7.

DMH2 ameliorates oxidative stress in an animal model of APAP- induced acute liver failure. A. mRNA levels of Hmox1, Gstm3, Sod2, Cat and Gpx1 determined by RT-qPCR and normalized to 36b4 gene expression. Data are expressed as fold increase relative to the control condition (C-DMSO, 1) and presented as mean ± SEM. B. GSH levels. Data are expressed as percentage relative to the control group (C-DMSO, 100%) and presented as mean ± SEM. C. Representative 20X and 40X images of 4-HNE IHC in liver sections and the corresponding quantification. Data are presented as mean ± SEM and expressed as arbitrary units (a.u.). D. Representative blots with the indicated antibodies and the corresponding quantification. Data are presented as mean ± SEM and expressed as percentage relative to control group (C-DMSO, 100%). Experimental conditions: Mice i. p. Injected with vehicle (DMSO) or APAP (500 mg/kg), and 1 h later treated with DMH2 or the corresponding amount of DMSO in a single dose of 3 mg/kg. Animals were sacrificed 2 h after APAP administration (n = 6 animals per group). *p < 0.05, **p < 0.01, ***p < 0.005 and ****p < 0.0001, APAP vs. C; ^#p < 0.05, ^##p < 0.01, ^###p < 0.005 and ^####p < 0.001, APAP-DMH2 vs. APAP-DMSO.