FIG. 4.

Human chromosome region 11p11.2-11cen activates p125 in hamster cells. (A) Binding activity. Extracts (2 μg) were assayed for p125 binding activity in three hybrid cell lines (lanes 5 to 10) derived from the DonTK− hamster line, which has a small amount of binding activity, and four hybrid cell lines (lanes 13 to 20) derived from the V79/380-6 hamster cell line, which does not have any detectable binding activity. (B) Human chromosomes contained in the hybrid cell lines. +, chromosome is present in at least 10% of the cells; P, part of the chromosome is present; P¹, 11p11.2-11qter is present; P², the reciprocal chromosome containing 11pter-11p11.2, is present; P³, 11pter-11cen is present. Discordance analysis was performed for the presence of each chromosome and activation of p125. The analysis included complete human chromosomes but not parts of chromosomes. Discordance was defined as either the absence of p125 activation in the presence of the chromosome or the presence of p125 activation in the absence of the chromosome. The percent discordance was calculated by dividing the number of discordant hybrids by the number of informative hybrids and multiplying by 100. F, free DNA probe migration; B1 and B2, UV damage-specific protein-DNA complex mobilities.