FIG. 7.
p125-p48 complex binds to UV-damaged DNA. (A) Coexpression of p125 with p48 increases the levels of both complexes. Expression vectors for p125 (p125, p125-HA, or FLAG-p125) and p48 (p48 or FLAG-p48) were transfected into 293T cell lines. Whole-cell extracts from the transfectants (2 μg) were assayed for damaged-DNA-specific binding activity with DNA probes damaged at a low dose (300 J/m2) of UV. (B) Whole-cell extracts (2 μg) were preincubated with anti-FLAG (F) or anti-HA (H) antibodies (0.02 μg/ml) at room temperature for 20 min before initiation of the binding reaction by addition of DNA probe damaged at the same dose of UV.