FIG. 8.

p125 interacts with p48 in free solution. Whole-cell extracts from 293T cells transfected with p125 (p125 or p125-HA) and FLAG-p48 were immunoprecipitated (IP) with anti-FLAG or anti-HA antibodies (Abs). The immunoprecipitated proteins (lanes 1 to 4) and 10 μg of whole-cell extract (lanes 5 to 7) were resolved by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was stained with Ponceau S to visualize the molecular weight markers and then cut into the two pieces. The top half, which contained proteins larger than 65 kDa, was immunoblotted (IB) with anti-HA antibodies, and the bottom half was immunoblotted with anti-FLAG antibodies.