FIG. 9.

p48 contains a WD motif homologous to the WD motif in a subfamily of proteins involved in chromatin reorganization. Homologous genes were identified by BLAST search and aligned by the BOXSHADE program. Identical amino acids are highlighted in black. Functionally conserved amino acids are highlighted in gray. Conserved amino acids are classified as follows: V, I, L, and M; D, E, Q, and N; F, Y, and W; G, S, T, P, and A; and K, R, and H. The positions of the mutated amino acids of p48 in XPE⁻ cells are marked as # (K-to-E change in 82TO) and ∗ (R-to-H change in 2415). The proteins are as follows: CAF-1 p48, chromatin assembly factor p48 (EMBL X74262) (44); RbA p46, retinoblastoma-associated protein (EMBL X72841) (34); CAF-1 p55 (GenBank U62388) (43); K07A1.12 and K07A1.11, identified by Caenorhabditis elegans genome sequencing (EMBL Z81097); XPE p48 (GenBank U18300) (12); CSA, Cockayne syndrome A protein (GenBank U28413) (18); YDL156w, identified by S. cerevisiae genome sequencing (EMBL Z74204); Msi1p, identified as a negative regulator of the Ras-cyclic AMP pathway (GenBank M27300) (39); and Hat2p, histone acetyltransferase (GenBank U18795) (32). The position of the first amino acid (a.a.) of each WD motif is shown to the left of the amino acid sequence, and the percent identity to p48, starting at the conserved His241, is shown to the right.