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. 2024 Feb 28;15:1799. doi: 10.1038/s41467-024-45751-9

Fig. 5. PC mitochondria display higher turnover via mitophagy.

Fig. 5

A mtKeima is a pH-sensitive mitophagy reporter. The ratio between the 440 nm (green; neutral pH) and 560 nm (red; acidic pH) excitation measures the proportion of mitochondria undergoing mitophagy. Illustration created using BioRender. B Intravital microscopy of transgenic mice expressing mtKeima in saline-injected or leupeptin-injected mice. Representative images of the hepatic lobule and magnified insets of PP and PC regions. C Quantification of mitophagy in PP and PC regions expressed as a fold-change relative to PP cells. Data presented as mean ± SD from n = 3 independent experiments. Statistical significance was calculated using two-way ANOVA, Tukey’s multiple comparisons test; ns not significant; *p = 0.03, **p = 0.001. D Immunoblots of Bnip3 and LC3A/B of unsorted (total) and sorted PP and PC hepatocyte populations from livers treated with either saline or leupeptin. Mouse 1 (M1); Mouse 2 (M2). E–F Quantification of Bnip3 expression and LC3B/A ratio. Data presented as mean ± SD from n = 5 (saline) or n = 6 (leupeptin) independent experiments. Statistical significance was calculated using two-way ANOVA, Tukey’s multiple comparisons tests; ns not significant; *p = 0.02 and **p = 0.006 (Bnip3) ;*p = 0.03 and **p = 0.0014 (LC3B/A). Periportal (PP) is labeled in red; pericentral (PC) in blue.