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. 2024 Feb 28;14:4841. doi: 10.1038/s41598-024-55356-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Compound features identified in polar and non-polar yeast extracts analyzed by positive and negative ion mode tandem mass spectrometry. Total number of compound features (MS¹ ions by m/z and retention time) are compared to those filtered from background ions, those normalized and filtered from background ions, and those normalized with MS² spectra and filtered from background ions. Polar extract solvents were methanol/water and non-polar extract solvents were chloroform/methanol.