TABLE 1.
Yeast strains used in this study
| Straina | Origin or source | Genotype |
|---|---|---|
| YME2b | 5 | MATa ade2-Δ1 his3-Δ187 leu2-Δ1 ura3-52 |
| YAF44 | 8 | rfa1-44; isogenic to YME2 |
| YAF5 | 8 | rad52-34; isogenic to YME2 |
| YAF22 | 8 | rad52-38; isogenic to YME2 |
| YAF100 | This study | rfa1-44 rad52-38; isogenic to YME2 |
| Y190 | 11 | MATa ade2-101 his3-Δ200 leu2-3,112 ura3-52 lys2-801 trp1-901 tyr1-501 canR gal4Δ gal80Δ cyhS URA3::GAL-lacZ LYS2::GAL-HIS3 |
All strains are heterothallic.
YME2 is a derivative of strain S288C.