TABLE 2.
Two-hybrid plasmids used in this study
| Plasmid | Source | Description |
|---|---|---|
| pAS1-CYH2 | 11 | Plasmid used for constructing fusions of proteins via their N termini to the DNA binding domain (DBD) of the Gal4 protein |
| pBG4D-1 | R. Brazas | Plasmid used for constructing fusions of proteins via their C termini to the DBD of the Gal4 protein |
| pGAD-GH | G. Hannon | Plasmid used for constructing fusions of proteins via their N termini to the transcriptional activation domain (TAD) of the Gal4 protein |
| pCAD-1 | 33 | Plasmid used for constructing fusions of proteins via their C termini to the TAD of the Gal4 protein |
| pSLH99 | 14 | GAL4-RAD51 TAD fusion expression vector made with pGAD-GH |
| pSLH127 | 14 | RAD52-GAL4 DBD fusion expression vector made with pBG4D |
| pSLH128 | This study | rad52-34–GAL4 DBD fusion expression vector made with pBG4D |
| pSLH129 | This study | rad52-38–GAL4 DBD fusion expression vector made with pBG4D |
| pSLH217 | This study | RAD52-GAL4 TAD fusion expression vector made with pCAD-1 |
| pSLH218 | This study | rad52-34–GAL4 TAD fusion expression vector made with pCAD-1 |
| pSLH219 | This study | rad52-38–GAL4 TAD fusion expression vector made with pCAD-1 |
| pSLH106 | 14 | GAL4-RAD55 TAD fusion expression vector made with pGAD-GH |
| pSLH108 | 14 | GAL4-RAD57 TAD fusion expression vector made with pGAD-GH |
| pSLH223 | This study | RFA1-GAL4 DBD fusion expression vector made with pBG4D-1 |
| pRB3 | This study | RFA2-GAL4 DBD fusion expression vector made with pBG4D-1 |
| pRB5 | This study | RFA3-GAL4 DBD fusion expression vector made with pBG4D-1 |