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. 2024 Mar;30(3):611–613. doi: 10.3201/eid3003.231160

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Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Phylogenetic analyses of Leishmania donovani from the ISC, including Nepal, and reference sequences. Trees were based on genomewide single-nucleotide polymorphisms using RAxML (8). A) Unrooted phylogenetic network of the L. donovani complex, showing samples representing the emerging foci (bold text). B) Rooted phylogenetic tree of reference strains of L. donovani from the ISC, showing the branching of 3 samples (022, 023, and 024) originating from emerging foci. Important bootstrap values are indicated on the branches. The West-African LV9 strain is included as an outgroup. BPK72_SuSL represents an ISC1 sample analyzed using SureSelect sequencing (Agilent Technologies; https://www.agilent.com), confirming that the branching of the emerging foci is not a result of a technical artifact. Scale bars indicate number of single-nucleotide polymorphism differences. ISC, Indian subcontinent.