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Determination of the growth of Acanthamoeba griffini trophozoites cultured in PYG medium with four different salt concentrations: 1 g/L (A), 5 g/L (B), 15 g/L (C) and 34 g/L (D), at 20, 28 and 37°C for 72 h, starting at a concentration of 100 cells/mL. Each data point represents the mean ± standard deviation (SD) of three different measurements. An increase of the cell population was observable at 1, 5 and 15 g/L after 72 h, while at 34 g/L the number of cells decreased.
