Figure 3.

Naegleria fowleri trophozoite growth in saline water at 1 (A), 5 (B) and 15 (C) g/L of salt incubated at 20, 26, 28 and 37°C. The number of cells was measured at 0, 24, 30, 48, 54 and 72 h. Each data point represents the average cell population of three different experiments ± SD. At 28°C the cellular concentration remained similar after 72 h of incubation at 1 and 5 g/L. Moreover, the number of cells persisted stable all along the experiments at 20°C and 1 g/L. Overall, the number of cells decreased, reaching to 0 viable cells in some cases, in the rest of the tested conditions.