Fig. 6.

Effects of Maplifa^™ and GA on MGO-induced production of mitochondria-derived ROS. HaCaT cells were treated with Maplifa^™ (50 μg/mL) or GA (50 μM) for 2 h, followed by exposure of MGO (400 μM) for 24 h. Next, the cells were incubated with 20 μM DCFDA for 20 mins. After the cells were washed, 5 μM MitoSOX^™ reagent working solution was added and incubated for 10 mins and the cells were assayed by flow cytometry (A). The frequency of ROS positive cells (B) and frequency of mitochondria-derived ROS positive cells were quantified (C) with FlowJo. ^#p < 0.05 and ^###p < 0.001 as compared with control; **p < 0.01 as compared with model goup.