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Effects of Maplifa™ and GA on MGO-induced production of mitochondria-derived ROS. HaCaT cells were treated with Maplifa™ (50 μg/mL) or GA (50 μM) for 2 h, followed by exposure of MGO (400 μM) for 24 h. Next, the cells were incubated with 5 μM MitoSOX™ reagent working solution for 10 mins and assayed by flow cytometry (A). Intensity of mitochondria-derived ROS measured by Tracker Red were quantified with FlowJo (B). #p < 0.05 and ###p < 0.001 as compared with control group.
