Table 2.
Biosynthesis of L-phenylalanine and trans-cinnamic acid by P. putida CM12-5 and P. putida CM12-5 (pPALN)
| [L-Phe] T 0 h (mg L−1) | [L-Phe] T 24 h (mg L−1) | [tCA] T 24 h (mg L−1) | |
|---|---|---|---|
| CM12-5 | 0 | 217.2 ± 41.8 | 0 |
| 173.2 ± 0.9 | 356.9 ± 5.5 | 0 | |
| CM12-5 (pPALN) | 0 | 141.8 ± 33.8 | 91.0 ± 11.2 |
| 173.6 ± 0.1 | 263.9 ± 41.0 | 119.7 ± 19.3 |
Assays were done in M9 minimal medium with glucose as the sole C-source with and without the addition of 1 mM L-Phe (173 ± 1 mg/L)
The concentrationof L-phenylalanine and trans-cinnamic acid was determined after 24 h’ cultivation
The data in the table are the average and standard deviation of three independent assays