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. 2024 Feb 28;23:69. doi: 10.1186/s12934-024-02341-0

Table 2.

Biosynthesis of L-phenylalanine and trans-cinnamic acid by P. putida CM12-5 and P. putida CM12-5 (pPALN)

[L-Phe] T 0 h (mg L−1) [L-Phe] T 24 h (mg L−1) [tCA] T 24 h (mg L−1)
CM12-5 0 217.2 ± 41.8 0
173.2 ± 0.9 356.9 ± 5.5 0
CM12-5 (pPALN) 0 141.8 ± 33.8 91.0 ± 11.2
173.6 ± 0.1 263.9 ± 41.0 119.7 ± 19.3

Assays were done in M9 minimal medium with glucose as the sole C-source with and without the addition of 1 mM L-Phe (173 ± 1 mg/L)

The concentrationof L-phenylalanine and trans-cinnamic acid was determined after 24 h’ cultivation

The data in the table are the average and standard deviation of three independent assays