Table 1.
Blockade by amitriptyline (%) |
||||||
---|---|---|---|---|---|---|
Construct(s) | n | Half-activation potential, V½ (mV) | Slope factor, k (mV/e-fold) | Current density (pA/pF) | 1 μM | 10 μM |
Kv7.2 | 28 | −22.3 ± 0.9 | 12.6 ± 0.6 | 26.9 ± 3.2 | – | – |
Kv7.2 Y141N | 22 | −35.4 ± 0.6∗ | 14.9 ± 0.6∗ | 50.1 ± 6.4∗ | ||
Kv7.2 G239S | 16 | −30.5 ± 1.7∗ | 14.3 ± 0.7∗ | 38.2 ± 5.5∗ | – | – |
Kv7.2 + Kv7.3 | 10 | −27.1 ± 1.0 | 10.6 ± 0.5 | 112.4 ± 15.1 | 8.1 ± 1.2 | 40.0 ± 3.8 |
Kv7.2 + Kv7.2 Y141N + Kv7.3 |
13 | −28.7 ± 1.1 | 11.3 ± 0.6 | 99.9 ± 17.0 | 9.7 ± 2.5 | 39.8 ± 3.0 |
Kv7.2 + Kv7.2 G239S + Kv7.3 |
11 | −28.7 ± 1.6 | 13.9 ± 1.0 | 135.5 ± 30.4 | 11.8 ± 3.6 | 64.6 ± 5.2∗∗ |
∗p < 0.05 versus Kv7.2; ∗∗p < 0.05 versus Kv7.2+Kv7.3.