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. 1998 Aug;18(8):4455–4462. doi: 10.1128/mcb.18.8.4455

FIG. 3.

FIG. 3

Dominant mutations in MED6 or SRB6 do not bypass a requirement for srb4-138. Shown are growth phenotypes of cells containing plasmid-borne copies of SRB4 or srb4-138, either alone or with the MED6-101 or SRB6-201 mutation. Cells were streaked on 5-FOA to select against URA3 versions of SRB4 or srb4-138 and incubated at 30°C for 2 days. Relevant genotypes are described as follows: 1, srb4Δ2::HIS3 [pCT127 (SRB4 LEU2 CEN)]; 2, srb4Δ2::HIS3 [pCT15 (SRB4 URA3 CEN)]; 3, srb4Δ2::HIS3 [pCT181 (srb4-138 LEU2 CEN)]; 4, srb4Δ2::HIS3 [pEG39 (srb4-138 URA3 CEN)]; 5, srb4Δ2::HIS3 MED6-101 [pEG39 (srb4-138 URA3 CEN)]; 6, srb4Δ2::HIS3 SRB6-201 [pEG39 (srb4-138 URA3 CEN)].