Fig. 3.

BMPER is a direct target of miR-145-5p. (A) Venn diagram describing the screened potential targets of miR-145-5p based on the RNA-Seq results and bioinformatic analyses. (B) Heatmap showing 24 overlapped genes. (C) PCR analyses of the expression level of BMPER in Y-ADSCs and O-ADSCs; n=9. (D) The sequences of BMPER-WT and BMPER-MUT were inserted into the psi-CHECK-2 vector and prediction of binding sequences between BMPER and miR-145-5p. (E) Luciferase activities of recombinant vectors with wildtype or mutated-type BMPER was examined in HEK-293 T cells by co-transfection with miR-145-5p mimic and mimic NC; n=3. (F) PCR analyses of the transfection efficiency of si-BMPER; n=3. (G) Western blotting analyses of BMPER expression in ADSCs after si-BMPER transfection; n=3. (H) Western blot analyses of BMPER expression in ADSCs after miR-145-5p mimic and miR-145-5p inhibitor transfection; n=3. Data were presented as mean±s.d.; unpaired Student's t-test was used in C and F. One-way ANOVA with Tukey's multiple comparisons test was used in E. ns, not significant; *P<0.05; **P<0.01; ***P<0.001.