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. 2022 Feb 1;3(1):011303. doi: 10.1063/5.0073494

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© 2022 Author(s).

Published under an exclusive license by AIP Publishing.

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FIG. 3. — A typical experimental setup for protein corona investigations. After incubation of nanoparticles in human blood plasma, nanoparticle–protein complexes are isolated by asymmetrical flow field-flow fractionation (AF4). The sizes can be assessed by dynamic light scattering (DLS). The protein corona composition and amount are analyzed by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and label-free quantitative proteomic analysis. LC-MS, liquid chromatography-mass spectrometry; UPLC, ultra-performance liquid chromatography. Reproduced with permission from Small 16, 1907574 (2020).²⁸ Copyright 2021 John Wiley and Sons.