FIG. 9.

Principle of the high-throughput barcoding system developed by Dahlman and co-workers. (a) Structure of the DNA barcodes with 8–10 central nucleotides (green) as barcode region. (b) Several hundred different barcoded nanoparticles (e.g., lipid nanoparticles, LNPs) are co-administrated in mice. With next-generation sequencing, the in vivo biodistribution of the distinct barcoded nanoparticles can be analyzed simultaneously. This technology is termed Joint Rapid DNA Analysis of Nanoparticles (JORDAN). (c) JORDAN does not allow for functional delivery screening, as this method does not discriminate between nanoparticles outside or inside the cells (left). Fast Identification of Nanoparticle Delivery (FIND) provides a remedy (right). Here, successfully transfected reporter cells are identified. Reproduced with permission from Adv. Healthcare Mater. 10, e2002022 (2021).³¹⁷ Copyright 2021 John Wiley and Sons.