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The low-glucose-induced conjugation of Δnrd1 cells is inhibited by exogenously added cAMP. Exponentially growing h90 nrd1+ and h90 Δnrd1 cells were cultured at 30°C for 48 h in PM medium containing 0.5% glucose, the indicated concentrations of cAMP, and 1 mM caffeine, and examined for conjugation efficiencies.
