TABLE 2.

Nrd1 function is independent of Pka1 and Cig2^a

Mutant	% Suppressionb after transformation with:
	pcL-x	pcLnrd1+	pcLcig2+	pcLpka1+
pat1-114	<0.04	14	61	39
pat1-114 Δnrd1	<0.07	9	72	37
pat1-114 Δcig2	<0.06	5	106	55
pat1-114 Δpka1	0.4	17	0.8	53

^aThe pat1-114, pat1-114 Δnrd1, pat1-114 Δcyc17, or pat1-114 Δpka1 mutant cells were transformed with the indicated plasmids and selected at the minimum restriction temperatures for each strain: 32.5°C for pat1-114, 32.2°C for pat1-114 Δnrd1, 31.8°C for pat1-114 Δcyc17, and 29.5°C for pat1-114 Δpka1. 

^bThe percent suppression was calculated by dividing the number of colonies formed at each restriction temperature by the number of colonies formed at 23°C.