FIG. 2.

Example of single-cell RNA sequencing and spatial transcriptomics workflows. (A) An illustration of droplet-based microfluidics single-cell RNA sequencing that consists of (1) dissociating a tissue or biological sample, (2) isolating single cells, unique molecular identifiers, and lysis buffer, (3) cell lysis, (4) mRNA capture and reverse transcription, (5) cDNA amplification, and (6) library construction. We describe this process in more detail in Sec. II A). (B) A visualization of the steps for next-generation sequencing-based spatial transcriptomic (as described in Sec. II B), which include (1) tissue preparation, staining, and imaging, (2) permeabilizing the tissue, (3) cDNA synthesis, amplification, and library construction, followed by (5) sequencing.