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. 2024 Feb 29;20(2):e1012033. doi: 10.1371/journal.ppat.1012033

Fig 1. RNS-dependent persisters are sensitive to ciprofloxacin.

Fig 1

(A) Representative fluorescence-activated cell sorting (FACS) plots of fluorescence dilution experiments tracking the proportion of growing (G; light gray) and non-growing (NG; dark gray) WT Salmonella recovered from WT macrophages (Mφ) in the presence (+AB) or in the absence (-AB) of cefotaxime at 16 h of infection. (B) 24 h cefotaxime and/or ciprofloxacin survival of WT Salmonella in WT Mφ normalized to values after 30 min internalization. p values are indicated (ANOVA with Tukey’s correction for multiple comparisons); error bars depict means and standard deviation (SD); n = 3. (C) 24 h cefotaxime (light blue) or ciprofloxacin (yellow) survival of WT Salmonella in unstimulated (-IFN-γ) or IFN-γ-stimulated (+IFN-γ) WT or Nos2-/- Mφ normalized to values after 30 min internalization. Distinction between RNS-dependent and independent persisters was determined using the proportion of persisters in Nos2-/- Mφ. p values are indicated (ANOVA with Dunnett’s correction for multiple testing against the–IFN-γ condition); error bars depict means and standard deviation (SD); n = 3. (D) Persister clearance after 24 h of cefotaxime or ciprofloxacin treatment following 24 h of cefotaxime treatment of WT Salmonella in unstimulated (-IFN-γ) or IFN-γ-stimulated (+IFN-γ) WT Mφ normalized to values after 30 min internalization. p values are indicated (unpaired t test at 48h); error bars depict means and standard deviation (SD). (E) 24 h cefotaxime (light blue) or ciprofloxacin (yellow) survival of the initial (Q285A) and recurrent (Q285F5) isolates of ST313 Salmonella Q285 in WT or Nos2-/- Mφ normalized to values after 30 min internalization. p values are indicated (ANOVA with Tukey’s correction for multiple comparisons); error bars depict means and standard deviation (SD); n = 3.