Table 1. Quality evaluation of samples from 3 batches.

	Library	Batch 1	Batch 2	Batch 3
Polymerase Reads	Polymerase Reads	5534582	6002833	7099439
	Polymerase Yield (GB)	427.02	540.46	410.25
	Polymerase Max Length	479619	493469	483234
	Polymerase Mean Length	77155.42	90033.59	57786.35
	Polymerase Read N50	154554	167339	130615
Subreads	Subread Yield (GB)	420.1	535.33	404.78
	Subreads Max Length	479619	493469	483234
	Subreads Mean Length	3001.47	4482.57	3127.49
	Subread N50	3557	5186	3508
CCS	CCS Reads	4412788	5079207	5914414
	CCS Yield (GB)	15.6	25.46	23.5
	CCS Max Length	27871	27593	27810
	CCS Mean Length	3534.33	5013.54	3973.65
	CCS N50 Length	4216	5791	4760
	CCS Mean Passes	27	19	17
	CCS MeanQV	0.96	0.97	0.94
Full-length (FL) Isoform Detection	All Paired	11184443	20967437	11008106
	FL	10013148	19427495	9427600
	Non-FL	206358	283797	283953
	Unknow	964937	1256144	1296552
	FL (%)	89.53	92.66	85.64
	Non-FL (%)	1.85	1.35	2.58
	Unknow (%)	8.63	5.99	11.78
	FL MeanLen	934.75	888.57	1081.15
	FL N50	1128	1044	1365
Cell Barcode (CB) Identification	FLNC	10013148	19427495	9427600
	CB in Whitelist	8858087	17083701	7834317
	CB in Whitelist (%)	88.46	87.94	83.1
	CB Correction	254281	553029	334615
	CB Correction (%)	2.54	2.85	3.55
	Total Corrected CB	9112368	17636730	8168932
	Total Corrected CB (%)	91	90.78	86.65