FIG. 1.

Effect of nutritional conditions and the snf1Δ mutation on IME1 transcript levels. Cells were assayed for the presence of IME1 and control (DED1) RNAs by S1 nuclease protection. (A) Log-phase wild-type yeast cells were transferred to different media for the indicated times. Lane 1, undigested probe at 10% of the amount used in the protection assays; lane 2, log-phase cells; lanes 3 to 5, sporulation medium; lanes 6 to 8, SC medium; lanes 9 to 11, YNB; lanes 12 to 14, 2% glucose; lanes 15 to 17, 0.5% ammonium sulfate; lanes 18 to 20, sporulation medium with 1% glucose. (B) Cells were transferred to sporulation medium (Sp) for the indicated times. Lanes 1 to 6, wild type; lanes 7 to 12, snf1Δ; lanes 13 to 18, snf1Δ plus YEp351-IME1. (C) Same as panel B, except that cells were transferred to medium containing only YNB (i.e, lacking a carbon source [−Carbon]) and the first lane contained undigested probe at 10% of the amount used in protection assays.