FIG. 2.
Effect of IME1 overexpression on DNA content in a snf1Δ mutant. To ensure that most cells started in G1, cultures were grown in SC medium lacking leucine for 36 h, sonicated, and incubated in the spent medium for a further 12 h. Cells were transferred to sporulation medium, and DNA content was analyzed by flow cytometry after 0 h (upper panels) or 24 h (lower panels). Peaks represent unreplicated (2C) or fully replicated (4C) DNA. (A) snf1Δ cells containing YEp351 (control plasmid); (B) snf1Δ cells containing YEp351-IME1.